Semi Prep HPLC Column, HPS Silica, 5um, 120A. 10mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
75001-SP100 Cogent HPS Silica™ Semi‑Prep HPLC Column is packed with high‑purity, unmodified silica, delivering consistent, reproducible performance for normal phase purification of polar and hydrophilic compounds.
Because the stationary phase is not chemically modified, it provides strong interaction with polar analytes, making it ideal for semi‑preparative purification of lipids, fats, and other hydrophilic compounds.
This proprietary HPS Silica™ phase has been widely used for many years as a premium-value alternative to commonly used silica columns such as Inertsil® and Luna®, offering dependable selectivity and long-term reproducibility.
The 5 µm particle size is well suited for semi‑prep applications, providing:
Lower backpressure vs smaller particles
Higher flow rate capability
Durable performance under repeated purification cycles
100 mm Length – Balanced Resolution for Semi‑Prep Purification
The 100 mm length provides a strong balance of resolution and throughput.
Higher resolution than 50 mm columns
Faster analysis than 150 mm or 250 mm formats
Increased peak capacity for moderately complex samples
✅ Recommended for routine purification and analytical-to-prep scale transfer
10 mm ID – Semi‑Preparative Scale
Designed for semi‑preparative purification workflows
Increased sample loading capacity
Suitable for scaling up analytical methods
Compatible with standard fraction collection systems
✅ Recommended for intermediate‑scale purification and method development
Flow Rate & System Requirements (10 mm ID, 5 µm)
⚠️ Requires semi‑preparative HPLC system capability
Typical flow rate range: ~4 – 12 mL/min
Low-to-moderate backpressure
Designed for higher flow purification
Compatible with preparative detectors and fraction collectors
✅ Important: A specialized HPLC system is required for columns of this size
Why Choose HPS Silica™ for Semi‑Prep
High‑Purity Unmodified Silica
Strong retention of polar compounds
Ideal for normal phase purification
USP L3 Compatibility
Suitable for compendial methods
Reliable alternative to legacy silica columns
Proven Performance
Widely used in analytical and preparative labs
Consistent batch-to-batch reproducibility
Performance Advantages
Low Backpressure (5 µm Particles)
Enables higher flow rates
Compatible with prep-scale systems
Fast Equilibration
Reduces purification cycle time
Strong Polar Retention
Ideal for lipids and hydrophilic compounds
Typical Applications
Semi‑preparative purification of lipids and fats
Normal phase separations
Polar compound isolation
Method scale‑up from analytical HPLC
Pharmaceutical and chemical purification workflows
Important Usage Notes
Designed for normal phase chromatography conditions
Use appropriate non‑polar mobile phases
Requires semi‑preparative HPLC system capability
Ensure proper equilibration for reproducible results
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable